Anti-alpha smooth muscle Actin Mouse mAb (100 μl)

Actin, aortic smooth muscle

Synonyms:

Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46, SMA, a-sma, sma

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human a-SMA

Uniprot ID:

P62736

Isotype:

lgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm, Cytoskeleton

Predicted MW. / Observed MW.

42 kDa / 42 kDa

WB

Human, Mouse, Rat

1: 500-1: 1000

heart, liver, lung

IHC/IF

Human

1: 500-1: 1500

skeletal muscle, endometrial carcinoma, colon cancer

WB analysis of a-SMA (GB12044).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of a-smA (GB12044).

Sample: Human skeletal muscle (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of a-smA (GB12044).

Sample: Human endometrial carcinoma (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

IHC analysis of a-smA (GB12044).

Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.