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Recombinant Anti-beta Actin Mouse mAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal ) |
| Gene Name: | Actin, cytoplasmic 1 |
Synonyms: | ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
Immunogen: | KLH conjugated synthetic peptidecorresponding to Mouse beta-Actin |
Uniprot ID: | P60710 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 45 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 3000-1: 5000 | lung, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | NIH/3T3, rectum, liver, colon, stomach |
ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549, HeLa. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.
Images
| WB analysis of beta Actin (GB15001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse rectum using β-Actin (GB15001) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using β-Actin (GB15001) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human colon using β-Actin (GB15001) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15001) at dilution of 1: 1000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed A549 cells using β-Actin (GB15001) at dilution of 1: 1000 |
| ICC analysis of beta Actin(GB15001) red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody:Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
