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Recombinant Anti-beta Tubulin Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Tubulin beta chain |
Synonyms: | Tubb5, Beta 5 tubulin, Class I beta tubulin, Tubulin beta class I, TUBB1, TUBB, TUBB5, Tubulin Beta-5 Chain, β tubulin, CDCBM6 |
Immunogen: | Recombinant protein corresponding to Human beta Tubulin |
Uniprot ID: | P07437 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. / Observed MW. | 50 kDa / 55 kDa |
Applications
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | brain, HeLa, Hep G2 |
ICC/IF | Human, Mouse, Rat | 1: 800-1: 1600 | HeLa, NIH3T3 |
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Images
| WB analysis of beta-Tubulin (GB15140). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| ICC analysis of beta-Tubulin (GB15140)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
| ICC analysis of beta-Tubulin (GB15140)red. Sample: NIH3T3, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Mouse IgG (H+L) (GB21301), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles.|
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
