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Anti-dUTPase Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | dUTPase rabbit polyclonal |
Gene Name: | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial |
Synonyms: | deoxyuridine triphosphatase, DUT, dUTP pyrophosphatase, dUTPase |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human dUTPase |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 27 kDa |
Observed MW. | 20kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1500 | spleen |
IHC/IF | Human, Rat | 1: 800-1: 1600 | Liver cancer, tonsil |
Background
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. T Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Images
| Western blot analysis of dUTPase (GB113791) at dilution of 1: 1500 |
| Immunohistochemistry analysis of paraffin-embedded human liver cancer using dUTPase (GB113791) at dilution of 1: 1600 |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using dUTPase (GB113791) at dilution of 1: 160 |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using dUTPase (GB113791) at dilution of 1: 1600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
