Reactivity: | H,M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | smooth muscle Myosin heavy chain 11 rabbit polyclonal antibody |
Gene Name: | Myosin-11 |
Synonyms: | Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, Myh11 |
Immunogen: | Recombinant protein corresponding to Mouse Myh11 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 227 kDa |
Observed MW. | 250 kDa |
Uniprot ID: | P35749, O08638, Q63862 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | uterus, ovary |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2800 | bladder cancer, brain, esophagus, liver cancer, lung |
Background
The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
Images
Western blot analysis of Myh11 (GB111220) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human bladder cancer using myh11 (GB111220) at dilution of 1: 1400 (400x lens) | |
Immunohistochemistry of paraffin embedded human liver cancer using myh11 (GB111220) at dilution of 1: 1400 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse lung using myh11 (GB111220) at dilution of 1: 1400 (400x lens) | |
Immunohistochemistry of paraffin embedded rat brain using myh11 (GB111220) at dilution of 1: 1400 (400x lens) | |
Immunohistochemistry of paraffin embedded rat esophagus using myh11 (GB111220) at dilution of 1: 1400 (400x lens) | |
Immunofluorescence of paraffin embedded mouse brain using myh11 (GB111220) at dilution of 1: 1300 (400x lens) | |
Immunofluorescence of paraffin embedded rat esophagus using myh11 (GB111220) at dilution of 1: 1300 (400x lens) | |
Immunofluorescence of paraffin embedded rat lung using myh11 (GB111220) at dilution of 1: 1300 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |