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By utilizing our innovative and proprietary lipid conjugation technology, PrimeFect™ Transfection Reagent is formulated by addition of a booster peptide, giving rise to up to 20 times higher efficiency on variety of mammalian cells. PrimeFect™ was shown to efficiently deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with less toxicity. The reagent, 1.0 ml, is sufficient for 667 transfections in 24 well plates or 333 transfections in 6 well plates.
Key Features
• Superior transfection efficiency for a broad range of cell lines.
• Does not require removal of serum or culture medium.
• Does not require washing or changing of medium after transfection.
• Low cytotoxicity.
Component
1. PrimeFect™ Transfection Reagent: 1 ml
Storage
Store at -20°C.
Case Study
Comparisons of Transfection Efficiency of PrimeFect™ Transfection Reagent with the Leading Brand Name Products
Assessment of PrimeFect™ transfection efficiencies using GFP visualization. 8 commonly-used cell lines were transfected with a GFP-expressing plasmid using PrimeFect™ transfection reagent. Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
Comparison of cytotoxicity between PrimeFect™, Lipofectamine 2000 and Lipofectamine 3000.
Figure A & B. Assessment of PrimeFect™ transfection efficiencies for siRNA. Hela cell line was transfected with either a GFP cDNA (A), or co-tranfected with GFP cDNA and an anti-GFP siRNA (B). Transfection efficiencies were assessed by GFP signal.
Figure C & D. Comparison of PrimeFect™ and Lipofectamine® 2000 in plasmid transfection on mouse neuronal cells. Mouse neuronal cells were transfected with a GFP-expressing plasmid using either PrimeFect™ transfection reagent (C), or Lipofectamine® 2000 (D). Cells were visualized 48 hours post-transfection under fluorescence microscopy (GFP filter).
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