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Anti-Prohibitin Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Prohibitin |
Synonyms: | B-cell receptor-associated protein 32, C-BAP 32, Phb, PHB, HEL-215, HEL-S-54e, PHB1, Prohibitin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Prohibitin |
Uniprot ID: | P67778 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Images
IHC/IF | Mouse, Rat | 1: 700-1: 1400 | brain, heart, small intestine |
ICC/IF | Mouse, Rat | 1: 100-1: 400 | NIH-3T3 |
Background
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis . This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription. Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis .
Images
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Immunohistochemistry of paraffin embedded mouse brain using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse heart using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse small intestine using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
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Immunohistochemistry of paraffin embedded rat small intestine using Prohibitin (GB111326) at dilution of 1:700 (400x lens) |
| ICC analysis of Prohibitin(GB111326)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
