Reactivity: | H,M,R & H,M |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | RAB21 rabbit polyclonal antibody |
Gene Name: | Ras-related protein Rab-21 |
Synonyms: | KIAA0118, Rab-12, member RAS oncogene family, RAB21 |
Immunogen: | Recombinantproteincorresponding to Mouse RAB21 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Golgi apparatus, Endosome, Endoplasmic reticulum |
Predicted MW. | 24 kDa |
Observed MW. | 24 kDa |
Uniprot ID: | Q9UL25, P35282, Q6AXT5 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, colon |
IHC/IF | Human, Mouse | 1: 500-1: 1500 | colon, liver |
Background
RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes.
Images
Western blot analysis of RAB21 (GB111663) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse colon using RAB21 (GB111663) at dilution of 1: 700 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse liver using RAB21 (GB111663) at dilution of 1: 700 (300x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |