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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | RAP1A rabbit polyclonal antibody |
Gene Name: | Ras-related protein Rap-1A |
Synonyms: | Rap1a, RAP1A, C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21, Member of RAS oncogene family |
Immunogen: | Recombinant protein corresponding to Mouse RAP1A |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. | 21 kDa |
Observed MW. | 21 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, cerebral cortex |
IHC/IF | Mouse, Rat | 1: 600-1: 3000 | skin, spleen, thymus |
Background
Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.
Images
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Western blot analysis of RaP1A (GB11893) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse spleen using Rap1A (GB11893) at dilution of 1: 1500 (300x lens) |
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Immunofluorescence of paraffin embedded mouse skin using Rap1A (GB11893) at dilution of 1: 1000 (200x lens) |
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Immunofluorescence of paraffin embedded mouse thymus using Rap1A (GB11893) at dilution of 1: 1000 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
