Your shopping cart is empty!
Anti-RPL27A Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 60S ribosomal protein L27a |
Synonyms: | L29, Rpl27a, ribosomal protein L27a, FLJ43464 |
Immunogen: | Recombinant protein corresponding to Mouse RPL27A |
Uniprot ID: | P14115 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, 293T, A549 |
ICC/IF | Human, Mouse | 1: 50-1; 200 | HeLa, NIH-3T3 |
Background
Rpl27a gene ontology annotations related to this gene include translation.
Images
| WB analysis of RPL27A(GB111782). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of RPL27A(GB111782)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPL27A(GB111782)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
