Your shopping cart is empty!
Anti-RPS10 Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S10 |
Synonyms: | Rps10, DBA9, S10, ribosomal protein S10 |
Immunogen: | Recombinant protein corresponding to Mouse RPS10 |
Uniprot ID: | P63325, P63326 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 19 kDa / 19 kDa |
Product Usage Information
WB | Mouse, Rat | 1:500-1:1000 | heart,spleen,kidney |
ICC/IF | Human, Mouse | 1: 50-1: 400 | Hela, NIH-3T3 |
Background
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S10 (RPS10) is a 165 amino acid component of the small ribosomal 40S subunit. While generally found in the cytoplasm, RPS10 can localize to the granular component (GC) region of the nucleolus. For this to occur, methylation by PRMT5 is required as well as an interaction with NPS1. Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9).
Images
| WB analysis of RPS10(GB111838). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of RPS10(GB111838)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RPS10(GB111838)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
