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| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Rab5 rabbit polyclonal antibody |
Gene Name: | Ras-related protein Rab-5A |
Synonyms: | Rab5a, NnyRab5a, Ubiquitin-protein ligase D3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Rab5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell membrane |
Predicted MW. | 24 kDa |
Observed MW. | 24 kDa |
Uniprot ID: | P20339, Q9CQD1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, testis |
IHC/IF | Human | 1: 500-1: 2000 | lung, lung cancer |
Background
Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes, and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nucleotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic.
Images
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Western blot analysis of RAB5A (GB111104) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human lung cancer using RAB5A (GB111104) at dilution of 1: 1000 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung using RAB5A (GB111104) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded human lung using RAB5A (GB111104) at dilution of 1: 500 |
| Immunofluorescence of paraffin embedded human colon using RAB5A (GB111104) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
