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| Reactivity: | H,M,R & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Ubiquitin-conjugating enzyme E2 A |
Synonyms: | E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, mHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, Ube2a, Rad6a |
Immunogen: | Recombinant protein corresponding to Mouse Rad6 |
Uniprot ID: | Q9Z255 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 17 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | placenta, brain, kidney |
ICC/IF | Human | 1: 300-1: 600 | HeLa |
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions.
Images
| WB analysis of Rad6(GB111539). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
|
ICC analysis of Rad6(GB111539)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
