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Anti-Renin Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Renin-1 |
Synonyms: | Angiotensinogenase, REN, Renin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Renin |
Uniprot ID: | P06281 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Secreted, Membrane |
Predicted MW. / Observed MW. | 44 kDa / 37 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 300-1: 500 | kidney |
IHC/IF | Mouse | 1: 200-1: 400 | kidney |
Background
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Images
| WB analysis of RENIN (GB115500). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Renin (GB115500). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0)(G1203), 98°C 20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
