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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | SAFB rabbit polyclonal antibody |
Gene Name: | Scaffold attachment factor B1 |
Synonyms: | SAF-B1, HAP, HET, HSP27 ERE TATA binding protein, SAF B, SAFB, SAFB1, scaffold attachment factor B, Scaffold attachment factor B1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse SAFB |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 105 kDa |
Observed MW. | 150 kDa |
Uniprot ID: | D3YXK2, O88453 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, testis, liver |
IHC/IF | Mouse, Rat | 1: 400-1: 800 | brain, liver, lung, testis |
Background
SAFB (scaffold attachment factor B) is a nuclear matrix-associated protein that binds to matrix- or scaffold-associating regions (MARs or SARs) on DNA and interacts with RNA polymerase II and serine-/arginine-rich RNA processing factors (SR proteins). SAFB, also designated HAP (hnRNP A1 associated protein) and HET (HSP 27-ERE-TATA-binding protein) is a proven hnRNP protein that has a speckled distribution in the nucleus and, in response to stress agents such as heat shock, is recruited to a few, large nuclear granules, called perichromatin granules.
Images
| Western blot analysis of SAFB (GB111932) at dilution of 1: 500 |
| Immunofluorescence of paraffin embedded mouse liver using SAFB (GB111932) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded mouse lung using SAFB (GB111932) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded rat brain using SAFB (GB111932) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat liver using SAFB (GB111932) at dilution of 1: 400 (400x lens) |
| Immunofluorescence of paraffin embedded rat testis using SAFB (GB111932) at dilution of 1: 400 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
