Reactivity: | M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | SERCA2 ATPase rabbit polyclonal antibody |
Gene Name: | Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 |
Synonyms: | SERCA2, SR Ca(2+)-ATPase 2, alcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, Atp2a2 |
Immunogen: | Recombinant protein corresponding to Mouse SERCA2 ATPase |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum |
Predicted MW. | 115 kDa |
Observed MW. | 115 kDa |
Uniprot ID: | O55143, P11507 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | heart |
IHC/IF | Mouse, Rat | 1: 250-1: 1000 | heart, skeletal muscle |
Background
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle.
Images
Western blot analysis of ATP2A2 (GB112371) at dilution of 1: 500 | |
Immunohistochemistry of paraffin embedded mouse heart using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse skeletal muscle using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) | |
Immunohistochemistry of paraffin embedded rat heart using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) | |
Immunohistochemistry of paraffin embedded rat skeletal muscle using ATP2A2 (GB112371) at dilution of 1: 500 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |