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Alpha-tubulin (ubiquitous) chain Rabbit pAb (50 μl)
| Applications: | WB, IHC, IF/IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | tubulin alpha 1b |
| Gene Symbol: | TUBA1B |
| Synonyms: | K-ALPHA-1; Alpha-tubulin (ubiquitous) chain |
| Gene ID: | 10376 |
| UniProt ID: | P68363 |
| Immunogen: | Recombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.72 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
| Immunofluorescence analysis of C6 cells using Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC024) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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