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Cy3-conjugated Goat anti-Mouse IgG (H+L) (200 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | Cy3 conjugated IgG |
| Conjugation: | Cy3. Ex:548nm. Em:562nm. |
| Immunogen: | Mouse IgG |
| Dilution: | IF/IC 1:50-1:200; FC 1:100-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.02 mg/mL |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Immunofluorescence analysis of HeLa cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. |
 | Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. |
 | Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (AC033, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L)(AS008, dilution 1:200) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. |
 | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4μg/mL,orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). Cy3 Goat Anti-Mouse IgG (H+L) (AS008, 1:200) was used as a secondary antibody. |
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