Your shopping cart is empty!
Applications: | IF/IC, FC |
Host Species: | Goat |
Isotype: | Cy3 conjugated IgG |
Conjugation: | Cy3. Ex:548nm. Em:562nm. |
Immunogen: | Rabbit IgG |
Dilution: | IF/IC 1:100-1:800; FC 1:100-1:800 |
Purification Method: | Affinity purification |
Concentration: | 1.02 mg/mL |
Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | Confocal imaging of U-2 OS cells using DNA topoisomerase I (TOP1) Rabbit mAb(A12409,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:100)(Red),ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076,dilution 1:200) (Green) |
![]() | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). Cy3 Goat Anti-Rabbit IgG (H+L)(AS007, 1:800) was used as a secondary antibody. |