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FITC-conjugated Donkey anti-Rabbit IgG (H+L) (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Donkey |
| Isotype: | Fluorescein conjugated IgG |
| Conjugation: | FITC. Ex:491nm. Em:516nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IF/IC 1:50-1:200; FC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/ml |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Immunofluorescence analysis of PC-12 cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Vimentin Rabbit mAb (A19607) at a dilution of 1:100 (40x lens). Secondary antibody: FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining. |
 | Flow cytometric analysis of Positive antibody E-Cadherin Rabbit mAb (2.5μg/mL) in various cells (orange) compare to Rabbit rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was FITC Donkey Anti-Rabbit IgG (H+L) (AS042) at 1:100. |
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