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FITC-conjugated Goat anti-Rabbit IgG, Fc fragment specific (100 μl)
| Applications: | IF/IC, FC |
| Host Species: | Goat |
| Isotype: | Fluorescein conjugated IgG |
| Conjugation: | FITC. Ex:491nm. Em:516nm. |
| Immunogen: | Rabbit IgG |
| Dilution: | IF/IC 1:100-1:500; FC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.5 mg/ml |
| Buffer: | PBS with 0.025% Sodium Azide,0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Confocal imaging of paraffin-embedded Mouse brain using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Confocal imaging of SH-SY5Y cells using βIII-Tubulin Rabbit mAb (A17913, dilution 1:200) followed by a further incubation with FITC F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific(AS083, dilution 1:500)(Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 μg/mL, blue line) or CD8A Rabbit mAb (A0663, 10 μg/mL orange line), followed by FITC conjugated goat anti-Rabbit pAb (AS083, 1:200 dilution) staining. Non-fluorescently stained Jurkat cells were used as blank control (red line). |
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