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| Applications: | WB, IHC, IF/IC, IP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Gene Name: | glyceraldehyde-3-phosphate dehydrogenase |
| Gene Symbol: | GAPDH |
| Synonyms: | G3PD; GAPD; HEL-S-162eP; GAPDH |
| Gene ID: | 2597 |
| UniProt ID: | P04406 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2). |
| Dilution: | WB 1:2000-1:10000; IHC 1:50-1:200; IF/IC 1:50-1:200; IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Purification Method: | Affinity purification |
| Concentration: | 3.59 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of various lysates using GAPDH Rabbit pAb (AC027) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
| Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb (AC027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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