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HRP-conjugated Mouse anti-Rabbit IgG Light Chain (100 μl)
| Applications: | WB, IP |
| Host Species: | Mouse |
| Isotype: | IgG1,Kappa |
| Conjugation: | HRP |
| Clone ID: | 6E9V4 |
| Immunogen: | Rabbit IgG |
| Dilution: | WB 1:5000-1:10000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.4 mg/mL |
| Buffer: | PBS with 0.75% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:10000 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg IKKα antibody (A2062). Western blot was performed from the immunoprecipitate using IKKα antibody (A2062) at a dilution of 1:1000. Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061) at 1:20000 dilution. |
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