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HRP conjugated Rabbit Anti-Mouse IgG1 mAb (100 μl)
| Applications: | ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Conjugation: | HRP |
| UniProt ID: | P01868 |
| Clone ID: | 3K6O5 |
| Immunogen: | Mouse IgG |
| Dilution: | ELISA 1:5000 -1:10000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.44 mg/mL |
| Buffer: | PBS with 0.05% proclin300,0.05% BSA,50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Dose response curve of HRP conjugated Mouse IgG1 mAb measured by ELISA. 1 µg/mL of various immunoglobulins were coated to 384-well plate, blank wells without protein were used as negative control (NC). The coated plate was blocked and subsequently incubated with 25 µL of HRP conjugated Rabbit Anti-Mouse IgG1 mAb in a 3 fold serial dilution from 1 μg/mL to 6.97×10^-2 pg/mL, incubation was performed at room temperature for 1 hour. The ELISA result demonstrated that Rabbit anti-Mouse IgG1 mAb has highly specific recognition of Mouse IgG1 while no or minimal cross reactivity to Mouse IgG 2a、Mouse IgG 2b、Mouse IgG3、Mouse IgM、Human IgG1、Rat IgG1. |
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