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| Reactivity: | M,R & H,M,R & H,M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Trophoblast glycoprotein |
Synonyms: | 5T4, 5T4 AG, 5T4 oncofetal antigen, M6P1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse 5T4 |
Uniprot ID: | Q9Z0L0 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 46 kDa / 43 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | heart |
IHC/IF | Human, Mouse, Rat | 1: 800-1: 2400 | stomach, kidney, spleen, lung, heart, testis, colon cancer |
ICC/IF | Human, Mouse | 1: 100-1: 400 | HeLa |
Background
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Images
| Western blot analysis of TPBG (GB114976) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human stomach using TPBG (GB114976) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded mouse kidney using TPBG (GB114976) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using TPBG (GB114976) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat lung using TPBG (GB114976) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat spleen using TPBG (GB114976) at dilution of 1: 800 |
| Immunofluorescence of paraffin embedded human colon cancer using TPBG (GB114976) at dilution of 1: 600 |
| Immunofluorescence of paraffin embedded mouse heart using TPBG (GB114976) at dilution of 1: 600 |
| Immunofluorescence of paraffin embedded rat testis using TPBG (GB114976) at dilution of 1: 600 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using TPBG (GB114976) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
