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| Reactivity: | M,R & M,R & M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase |
Synonyms: | Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase, Alg6, My046, Asparagine linked glycosylation 6, Asparagine-linked glycosylation protein 6 homolog |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ALG6 |
Uniprot ID: | Q3TAE8 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum |
Predicted MW. / Observed MW. | 58 kDa / 50 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | liver, heart, placenta |
IHC/IF | Mouse, Rat | 1: 600-1: 1200 | placenta, hippocampus |
ICC/IF | Mouse, Rat | 1: 50-1: 300 | NIH-3T3 |
Background
Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol .
Images
| Western blot analysis of ALG6 (GB112532) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse placenta using ALG6 (GB112532) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin-embedded rat hippocampus using ALG6 (GB112532) at dilution of 1: 1200 |
| ICC analysis of ALG6(GB112532)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
