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Anti-ALKBH1 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Nucleic acid dioxygenase ALKBH1 |
Synonyms: | ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ALKBH1 |
Uniprot ID: | P0CB42 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 44 kDa / 42 kDa |
Product Usage Information
WB | Human | 1: 800-1: 1500 | Jurkat |
Background
Dioxygenase that acts as on nucleic acids, such as DNA and tRNA . Requires molecular oxygen, alpha-ketoglutarate and iron . A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment. Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs . Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation.
Images
| Western blot analysis of ALKBH1 (GB114740) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
