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Anti-ALKBH3 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 |
Synonyms: | ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ALKBH3 |
Uniprot ID: | Q8K1E6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Product Usage Information
IHC/IF | Human | 1: 400-1: 800 | liver cancer, breast cancer |
Background
Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation . Has a strong preference for single-stranded DNA . Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.
Images
| Immunohistochemistry analysis of paraffin embedded human liver cancer using ALKBH3 (GB114741) at dilution of 1: 400 |
| Immunohistochemistry analysis of paraffin embedded human breast cancer using ALKBH3 (GB114741) at dilution of 1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
