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Anti-ATP6V1E1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATP6V1E1 rabbit polyclonal antibody |
Gene Name: | V-type proton ATPase subunit E 1 |
Synonyms: | ATP6E, ATP6E2, ATP6V1E, ATP6V1E1, P31, V ATPase 31 kDa subunit, V ATPase subunit E 1, Vma4 |
Immunogen: | Recombinant protein corresponding to Mouse ATP6V1E1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. | 26 kDa |
Observed MW. | 35 kDa |
Uniprot ID: | P50518 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 3000-1: 6000 | brain |
IHC/IF | Human, Rat | 1: 1000-1: 2000 | kidney, pancreas |
Background
ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.
Images
 | Western blot analysis of ATP6V1E1 (GB114036) at dilution of 1: 5000 |
 | Immunohistochemistry of paraffin embedded human kidney using ATP6V1E1 (GB114036) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded human pancreas using ATP6V1E1 (GB114036) at dilution of 1: 4000 |
 | Immunohistochemistry of paraffin embedded rat pancreas using ATP6V1E1 (GB114036) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded rat brain using ATP6V1E1 (GB114036) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded rat kidney using ATP6V1E1 (GB114036) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
