Your shopping cart is empty!
Anti-Alas1 Rabbit pAb (100 μl)
| Reactivity: | H & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 5-aminolevulinate synthase, nonspecific, mitochondrial |
Synonyms: | ALAS, ALAS H, ALAS1, ALAS3, ALASH, Delta ALA synthase 1, MIG4, Mitochondrion Marker |
Immunogen: | Recombinant protein corresponding to Mouse ALAS1 |
Uniprot ID: | Q8VC19 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 71 kDa / 65 kDa |
Product Usage Information
WB | Human | 1: 300-1: 500 | A549 |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H.
Images
| Western blot analysis of ALAS1 (GB114365) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using Alas1(GB114365) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
