Your shopping cart is empty!
Anti-Aquaporin 4 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Aquaporin-4 |
Synonyms: | AQP4, Aquaporin 4, HMIWC2, MIWC, WCH4, Aquaporin type 4, Mercurial-insensitive water channel, aquaporin 4 |
Immunogen: | Recombinant protein corresponding to Mouse Aquaporin 4 |
Uniprot ID: | P55088 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 600-1: 2000 | brain, colon, kidney |
Background
Aquaporin-4, also known as AQP4, is a water channel protein, belonging to the aquaporin family of integral membrane proteins that conduct water through the cell membrane. In the CNS, AQP4 is the most prevalent aquaporin channel, specifically located at the perimicrovessel astrocyte foot processes, glia limitans, and ependyma. Aquaporin-4’s overall function is to provide fast water transportation as well as maintain homeostatic balance within the central nervous system. Other systems are also regulated by AQP4.
Images
| IHC analysis of AQP4 (GB11529). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IHC analysis of AQP4 (GB11529). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IHC analysis of AQP4 (GB11529). Sample: Rat brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IHC analysis of AQP4 (GB11529). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
 |
IF analysis of AQP4 (GB11529). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
 |
IF analysis of AQP4 (GB11529). Sample: Mouse colon (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of AQP4 (GB11529). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
 |
IF analysis of AQP4 (GB11529). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
 |
IF analysis of AQP4 (GB11529). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IHC analysis of AQP4(GB11529). Sample: Human glioblastoma multiforme,4% PFA (G1101) 12-24h. Antigen retrieval:citrate buffer (pH 6.0) (G1201)， pressure cooker 2min. Primary antibody:1: 600, 4°C overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of AQP4(GB11529). Sample: Human stomach,4% PFA (G1101) 12-24h. Antigen retrieval:citrate buffer (pH 6.0) (G1201)， pressure cooker 2min. Primary antibody:1: 600, 4°C overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of AQP4(GB11529). Sample: Human lung,4% PFA (G1101) 12-24h. Antigen retrieval:citrate buffer (pH 6.0) (G1201)， pressure cooker 2min. Primary antibody:1: 600, 4°C overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
