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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Bag3 rabbit polyclonal antibody |
Gene Name: | BAG family molecular chaperone regulator 3 |
Synonyms: | BAG3, BAG-3, BIS, CAIR-1, MFM6, BCL2 |
Immunogen: | Recombinant protein corresponding to Human Bag3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 62 KDa |
Observed MW. | 86 KDa |
Uniprot ID: | O95817, Q9JLV1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, muscle, brain |
Background
BAG proteins compete with Hip-1 for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Images
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Western blot analysis of Bag3 (GB11536) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
