Anti-BrdU Mouse mAb (100 μl)
-23%

Anti-BrdU Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB12051-100
Availability: In Stock
Special price:$199.00
Old price:$259.00
You save:$60.00
-+
Reactivity: All species    
Applications: IHC/IF    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Bromodeoxyuridine

Synonyms:

Bromodeoxyuridine, Bromodeoxyuridine,5-Bromo-2′-deoxyuridine; 5-BrdU, Proliferation Marker; 5-Bromo-2-deoxyUridine

Immunogen:

KLH conjugated Synthetic peptide corresponding to Mouse BrdU

Isotype:

IgG1,κ

Purity:

Affinity purification

Subcellular location:

Nucleus




Product Usage Information

IHC/IF

All species

1: 100-1: 500

jejunum, neonatal mouse's brain




Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it.




Images

Anti-BrdU Mouse mAb

IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water,37°C, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

IHC analysis of Brdu (GB12051).
Sample: Mouse jejunum (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: 2M HCL in pure water,37°C, 30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

IF analysis of Brdu (GB12051).
Sample: Mouse neonatal mouse's brain (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-mouse IgG (H+L)(GB21301),1: 200 RT 1h.




Storage

StorageStore at -20 °C for one year. Avoid repeated freeze/ thaw cycles.
Storage BufferPBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.


Related Products


LifeSct © 2025

Publications

text_heading

Our Location

405 East Gude Dr., Suite 205, Rockville, MD 20850, USA

1-301-890-6420