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| Reactivity: | H & H & H |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CD14 rabbit polyclonal |
Gene Name: | Monocyte differentiation antigen CD14 |
Synonyms: | |
Immunogen: | Recombinant human CD14 protein expressed in HEK293 cells |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Secreted, Golgi apparatus |
Predicted MW. | 40 kDa |
Observed MW. | 50-60 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | THP-1, Raji |
IHC | Human | 1: 500-1: 1000 | appendix, spleen, tonsil |
IF | Human | 1: 500-1: 1000 | tonsil, lung |
Background
CD14 is a 50-55 kDa glycosylphosphatidylinositol anchored glycoprotein. CD14 was preferentially expressed in monocytes and macrophages. CD14 acts as a common receptor of lipopolysaccharide (LPS) (together with TLR4 and MD-2). It plays an important role in the inflammatory response of monocytes to LPS.
Images
| Western blot analysis of CD14 (GB113374) at dilution of 1: 1000 Lane1: THP-1 cell lysate Lane2: Raji cell lysate Lane3-6: Recombinant antigen protein lysate in different dilution gradient |
| Immunohistochemistry analysis of paraffin-embedded human appendix using CD14 (GB113374) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human spleen using CD14 (GB113374) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using CD14 (GB113374) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human lung using CD14 (GB113374) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human tonsil using CD14 (GB113374) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
