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Anti-CD163 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Scavenger receptor cysteine-rich type 1 protein M130 |
Synonyms: | CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163, SCARI1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CD163 |
Uniprot ID: | Q86VB7 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | Cell membrane, Secreted |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Human | 1: 500-1: 1000 | liver cancer, lung, tonsil |
Background
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Images
| IHC analysis of CD163 (GB113152). Sample: Human liver cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD163 (GB113152). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD163 (GB113152). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
