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Recombinant Anti-CD31 Rabbit mAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Platelet endothelial cell adhesion molecule |
Synonyms: | CD31 Antigen, EndoCAM, PECAM-1, GPIIA, PECA1, CD31 |
Immunogen: | Recombinant protein corresponding to Human CD31 |
Uniprot ID: | P16284 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Human | 1: 200-1: 400 | Kidney, placenta, colon cancer |
Background
Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that in humans is encoded by the PECAM1 gene found on chromosome 17. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation.
Images
| IHC analysis of CD31(GB153151). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: S-Vision ploy conjugated goat Anti-Rabbit IgG(H+L),Ready to use(G1312-1), RT, 20min.
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| IHC analysis of CD31(GB153151). Sample: Human kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: S-Vision ploy conjugated goat Anti-Rabbit IgG(H+L),Ready to use(G1312-1), RT, 20min. |
| IHC analysis of CD31(GB153151). Sample: Human liver cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: S-Vision ploy conjugated goat Anti-Rabbit IgG(H+L),Ready to use(G1312-1), RT, 20min. |
| IHC analysis of CD31(GB153151). Sample: Human placenta, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: S-Vision ploy conjugated goat Anti-Rabbit IgG(H+L),Ready to use(G1312-1), RT, 20min. |
| IHC analysis of CD31(GB153151). Sample: Human renal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: S-Vision ploy conjugated goat Anti-Rabbit IgG(H+L),Ready to use(G1312-1), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
