Recombinant Anti-CD34 Rabbit mAb (100 μl)

Recombinant Anti-CD34 Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB153798-100
Availability: In Stock
$259.00
-+
Reactivity: H    
Applications: IHC/IF    
Host Species: Rabbit    
Clonality: Monoclonal    
Gene Name:

Hematopoietic progenitor cell antigen CD34

Synonyms:

CD34, HPCA1, Mucosialin, Cluster designation 34, cluster of differentiation 34, CD34 antigen, CD34 molecule

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human CD34

Uniprot ID:

P28906

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cell membrane




Product Usage Information

Applications

Species

Dilution

Positive Tissue

IHC/IF

Human

1: 500-1: 1000

Kidney




Background

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources.




Images

01 GB153798_CD34_ZCR240231215_ZCR240231215_d500_H kidney_20.0x_IHC_WM.png

IHC analysis of CD34 (GB153798).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB153798_CD34_ZCR240231215_ZCR240231215_d500_H kidney_20.0x_IHC_WM.png


IHC analysis of CD34 (GB153798).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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