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| Reactivity: | M & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | H-2 class II histocompatibility antigen gamma chain |
Synonyms: | Ia antigen-associated invariant chain, Ii, MHC class II-associated invariant chain, CD74 |
Immunogen: | Recombinant protein corresponding to Mouse CD74 |
Uniprot ID: | P04441 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 30 kDa / 30-40 kDa |
Product Usage Information
WB | Mouse | 1: 300-1: 600 | spleen, lymph node |
IHC/IF | Mouse | 1: 500-1: 1000 | lymph node, spleen, model of antigen stimulation spleen, lung injury |
Background
CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized.
Images
| Western blot analysis of CD74 (GB115175) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded mouse lymph node using CD74 (GB115175) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using CD74 (GB115175) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse model of antigen stimulation spleen using CD74 (GB115175) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded mouse lung injury using CD74 (GB115175) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
