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Anti-COX IV Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Synonyms: | Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1 |
Immunogen: | Recombinant protein corresponding to Mouse COX IV |
Uniprot ID: | P19783 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion inner membrane |
Predicted MW. / Observed MW. | 20 kDa / 17 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 750-1: 1500 | heart, kidney |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 800 | gastric cancer, heart, kidney |
ICC/IF | Human, Mouse, Rat | 1: 50-1: 200 | NIH-3T3 |
IEM | Mouse | 1: 50-1: 100 | heart muscle |
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2.
Images
| Western blot analysis of COX IV (GB11250) at dilution of 1: 750 |
 | Immunohistochemistry of paraffin embedded human gastric cancer using COX IV (GB11250) at dilution of 1: 400 (200x lens) |
| Immunohistochemistry of paraffin embedded human liver cancer using COX IV (GB11250) at dilution of 1: 400 (300x lens) |
| Immunohistochemistry of paraffin embedded mouse heart using COX IV (GB11250) at dilution of 1: 400 (250x lens) |
| Immunohistochemistry of paraffin embedded mouse liver using COX IV (GB11250) at dilution of 1: 400 (300x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using COX IV (GB11250) at dilution of 1: 400 (200x lens) |
| Immunohistochemistry of paraffin embedded rat liver using COX IV (GB11250) at dilution of 1: 400 (300x lens) |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50 . A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse myocardium using COX IV (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunofluorescence of paraffin embedded human colon using COXIV (GB11250) at dilution of 1: 200 |
| Immunofluorescence of paraffin embedded mouse heart using COXIV (GB11250) at dilution of 1: 200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using COX IV(GB11250) at dilution of 1:50-1:200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using COX IV(GB11250) at dilution of 1:50-1:200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
