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| Reactivity: | H & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CSK rabbit polyclonal antibody |
Gene Name: | Tyrosine-protein kinase CSK |
Synonyms: | C-Src kinase, Protein-tyrosine kinase MPK-2, p50CSK, Csk |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CSK |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. | 51 kDa |
Observed MW. | 51 kDa |
Uniprot ID: | P41240 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 250-1: 500 | Jurkat, A549, Raji |
IHC/IF | Mouse, Rat | 1: 400-1: 800 | lung, spleen |
Background
All members of the Src gene family of tyrosine kinases are characterized by a carboxy terminal domain tyrosine which is highly phosphorylated in the inactive form of the enzyme and phosphorylated to a much lesser extent when the enzyme is active. In the case of Src p60, Y527 is this tyrosine; however, a mutant form of c-Src in which Y527 is replaced by phenylalanine is transforming and displays 5- to 10-fold elevated kinase activity compared to its normal counterpart. Csk has been identified as a Src-related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences amino terminal to the SH3 domain as well as carboxy terminal regulatory sequences.
Images
 | Western blot analysis of Csk (GB112489) at dilution of 1: 250 |
 | Immunofluorescence of paraffin embedded mouse lung using CSK (GB112489) at dilution of 1: 400 (200x lens) |
 | Immunofluorescence of paraffin embedded mouse spleen using CSK (GB112489) at dilution of 1: 400 (400x lens) |
 | Immunofluorescence of paraffin embedded rat spleen using CSK (GB112489) at dilution of 1: 400 (450x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
