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Recombinant Anti-Calbindin Mouse mAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Calbindin |
Synonyms: | Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1, CAB27, CALB1, CALB, Calbindin 1 |
Immunogen: | Recombinant protein corresponding to Mouse Calbindin |
Uniprot ID: | P12658 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 30 kDa / 30 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | kidney, brain |
IHC/IF | Mouse, Rat | 1: 250-1: 1000 | cerebellum |
Background
Calbindin 1 is a protein that in humans is encoded by the CALB1 gene. The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.
Images
| WB analysis of Calbindin(GB15668). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Calbindin(GB15668). Sample: Mouse cerebellum, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Calbindin(GB15668). Sample: Mouse kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Calbindin(GB15668). Sample: Rat cerebellum, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Calbindin(GB15668). Sample: Rat kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
