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| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Serine/ threonine-protein kinase Chk2 |
Synonyms: | CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, checkpoint kinase 2 |
Immunogen: | Recombinant protein corresponding to Mouse Chk2 |
Uniprot ID: | Q9Z265 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Applications
Applications | Species | Dilution | Positive Sample |
IHC/IF | Human | 1: 100-1: 1000 | colon, colon cancer, stomach cancer |
Background
CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs). Thus, CHK2’ s inhibition of the CDC25 phosphatases prevents entry of the cell into mitosis.
Images
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Immunohistochemistry of paraffin embedded human colon cancer using CHK2 (GB12575) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon using CHK2 (GB12575) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded human stomach cancer using CHK2 (GB12575) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
