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Anti-Cofilin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Cofilin rabbit polyclonal antibody |
Gene Name: | Cofilin-1 |
Synonyms: | Cofilin, non-muscle isoform, Cfl1, HEL-S-15, epididymis secretory protein Li 15, p18 |
Immunogen: | Recombinant protein corresponding to Mouse Cofilin/KLH conjugated Synthetic peptide corresponding to Mouse Cofilin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Nucleus |
Predicted MW. | 19 kDa |
Observed MW. | 19 kDa |
Uniprot ID: | P23528, P18760, P45592 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver |
IHC/IF | Human, Mouse, Rat | 1: 650-1: 6500 | liver, lung, lung cancer, ovarian cancer, ovary |
Background
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Images
| Western blot analysis of cofilin (GB112496) at dilution of 1: 500 |
 | Immunofluorescence of paraffin embedded human lung cancer using cofilin (GB112496) at dilution of 1: 6500 (250x lens) |
 | Immunofluorescence of paraffin embedded human ovarian cancer using cofilin (GB112496) at dilution of 1: 650 (200x lens) |
 | Immunofluorescence of paraffin embedded mouse liver using cofilin (GB112496) at dilution of 1: 650 (200x lens) |
 | Immunofluorescence of paraffin embedded mouse lung using cofilin (GB112496) at dilution of 1: 650 (200x lens) |
| Immunofluorescence of paraffin embedded rat ovary using cofilin (GB112496) at dilution of 1: 650 (300x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
