Reactivity: | M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Collagen V rabbit polyclonal antibody |
Gene Name: | Collagen alpha-1(V) chain |
Synonyms: | Col5a1, Collagen Type V, Alpha 1 type V collagen, Col5A2, Col5A3, Collagen alpha 2(V) chain, Collagen type V alpha 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Collagen V |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. | 184 kDa |
Observed MW. | 184 kDa |
Uniprot ID: | O88207, Q9JI03 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | liver |
IHC/IF | Mouse, Rat | 1: 750-1: 3000 | heart, kidney, lung, skin |
Background
COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Images
Western blot analysis of collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse heart using collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 1800 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse kidney using collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 1800 (400x lens) | |
Immunohistochemistry of paraffin embedded mouse lung using collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 1800 (400x lens) | |
Immunohistochemistry of paraffin embedded rat heart using collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 1800 (400x lens) |
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Immunofluorescence of paraffin embedded rat skin using collagen alpha-1 (Ⅴ) (GB111012) at dilution of 1: 1500 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |