Your shopping cart is empty!
Recombinant Anti-Cytokeratin 14 Mouse mAb (100 μl)
| Reactivity: | H & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Keratin, type I cytoskeletal 14 |
Synonyms: | Krt14 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 14 |
Uniprot ID: | Q61781 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 53 kDa / 53 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | A-431 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | esophageal carcinoma,skin |
Background
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Images
| WB analysis of CK14 (GB15803). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG(GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: esophageal carcinoma,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:1000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: Mouseskin,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1:1000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
| IHC analysis of Cytokeratin 14(GB15803). Sample: Ratskin,4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min . Primary antibody: 1:1000, 4°Covernight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
