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| Reactivity: | M,R & M,R |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | DAK rabbit polyclonal |
Gene Name: | Triokinase/FMN cyclase |
Synonyms: | DAK, DHA kinase, FMN cyclase, NET45 |
Immunogen: | Recombinant protein corresponding to Mouse DAK |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 60 kDa |
Observed MW. | 60 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | liver, kidney |
IHC | Mouse, Rat | 1: 2500-1: 5000 | kidney, brain, small intestine, ileum, jejuno |
Background
DAK, also name as TKFC, Triokinase, and FMN cyclase, catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. DAK has some isoforms with MW 51-55 kDa and 59 kDa.
Images
| Western blot analysis of DAK (GB113139) at dilution of 1: 1000 Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse kidney using DAK (GB113139) at dilution of 1: 5000 |
| Immunohistochemistry analysis of paraffin-embedded rat kidney using DAK (GB113139) at dilution of 1: 5000 |
| Immunohistochemistry analysis of paraffin-embedded mouse brain using DAK (GB113139) at dilution of 1: 5000 |
| Immunohistochemistry analysis of paraffin-embedded mouse small intestine using DAK (GB113139) at dilution of 1: 5000 |
| Immunohistochemistry analysis of paraffin-embedded rat ileum using DAK (GB113139) at dilution of 1: 5000 |
| Immunohistochemistry analysis of paraffin-embedded rat jejuno using DAK (GB113139) at dilution of 1: 5000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
