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Anti-DGAT1 Rabbit pAb (100 μl)
| Reactivity: | H & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Diacylglycerol O-acyltransferase 1 |
Synonyms: | ACAT related gene product 1, AGRP1, ARGP1, DGAT, DGAT1, Diglyceride acyltransferase |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse DGAT1 |
Uniprot ID: | Q9Z2A7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum membrane |
Predicted MW. / Observed MW. | 57 kDa / 50 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa, MCF7, Hep G2 |
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | duodenum |
Background
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates . Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats . In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage . Also present in female mammary glands, where it produces fat in the milk . May be involved in VLDL (very low density lipoprotein) assembly . In contrast to DGAT2 it is not essential for survival .
Images
| Western blot analysis of DGAT1 (GB114597) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse duodenum using DGAT1 (GB114597) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat duodenum using DGAT1 (GB114597) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded mouse duodenum using DGAT1 (GB114597) at dilution of 1: 1000 |
| Immunofluorescence analysis of paraffin embedded rat duodenum using DGAT1 (GB114597) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
