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| Reactivity: | |
| Applications: | WB & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Enhanced GFP |
Synonyms: | Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
Immunogen: | Recombinant protein corresponding to EGFP(enhanced green fluorescent protein) |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Product Usage Information
WB | Species independent | 1: 500-1: 1000 | cells/ tissues with eGFP |
ICC/IF | Species independent | 1: 2000-1: 4000 | CHO-S |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.
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Images
| Western blot analysis of EGFP (GB12602) at dilution of 1: 1000 |
 | Immunocytochemistry analysis of paraffin embedded CHO-S using eGFP (GB12602) at dilution of 1: 1500 Blue: DAPI Red: Anti-eGFP (Cy3) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
