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| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ETFA rabbit polyclonal antibody |
Gene Name: | Electron transfer flavoprotein subunit alpha, mitochondrial |
Synonyms: | Alpha-ETF, EMA, ETFA, GA2, MADD |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ETFA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Predicted MW. | 35 kDa |
Observed MW. | 35 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | liver, kidney, small intestine |
IHC/IF | Human | 1: 800-1: 3000 | kidney, colon |
Background
Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.
Images
 | Western blot analysis of ETFA (GB113834) at dilution of 1: 2000 |
 | Immunohistochemistry analysis of paraffin-embedded human kidney using ETFA (GB113834) at dilution of 1: 3000 |
 | Immunofluorescent analysis of paraformaldehyde-fixed human colon using ETFA (GB113834) at dilution of 1: 3000 |
 | Immunofluorescent analysis of paraformaldehyde-fixed human kidney using ETFA (GB113834) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
