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| Reactivity: | M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Electron transfer flavoprotein subunit beta |
Synonyms: | Beta ETF, ETFB, FP585, MADD |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ETFB |
Uniprot ID: | Q9DCW4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 28 kDa / 28 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 2000-1: 4000 | heart, liver, brain, skeletal muscle |
ICC/IF | Human, Mouse, | 1: 100-1: 300 | NIH-3T3 |
Background
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase . Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism . ETFB binds an AMP molecule that probably has a purely structural role .
Images
| WB analysis of ETFB(GB114623). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of ETFB(GB114623)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
